Distribution and frequency of tomato ringspot virus (ToRSV) in different varieties of Rubus idaeus in the Maule Region, Chile

The raspberry (Rubus idaeus) is one of the most important fruit for production in the Maule Region, Chile. Raspberries are affected by the tomato ringspot virus (ToRSV), which causes decreased yield and deformed fruit. The objective of this work is to study ToRSV spread in different raspberry variet...

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Autores principales: González Silva, Gloria Rossana, Concha Espinoza, Cynthia Macarena, Valenzuela Bustamante, Myriam Andrea, Cordero Alday, Luzmira Cecilia, Pico Mendoza, José Newthon, Cáceres Ruz, Pablo Alfredo, García González, Rolando
Formato: Artículo
Lenguaje:Inglés
Publicado: Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias 2017
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Acceso en línea:http://bdigital.uncu.edu.ar/9323
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author González Silva, Gloria Rossana
Concha Espinoza, Cynthia Macarena
Valenzuela Bustamante, Myriam Andrea
Cordero Alday, Luzmira Cecilia
Pico Mendoza, José Newthon
Cáceres Ruz, Pablo Alfredo
García González, Rolando
author_facet González Silva, Gloria Rossana
Concha Espinoza, Cynthia Macarena
Valenzuela Bustamante, Myriam Andrea
Cordero Alday, Luzmira Cecilia
Pico Mendoza, José Newthon
Cáceres Ruz, Pablo Alfredo
García González, Rolando
author_sort González Silva, Gloria Rossana
collection Repositorio
description The raspberry (Rubus idaeus) is one of the most important fruit for production in the Maule Region, Chile. Raspberries are affected by the tomato ringspot virus (ToRSV), which causes decreased yield and deformed fruit. The objective of this work is to study ToRSV spread in different raspberry varieties in the Maule Region, Chile. The virus was detected using the ELISA test and RT-PCR in the Heritage, Meeker, Chilliwack, Amity and Coho varieties. Bayesian analysis determined the relationship between the percentage of ToRSV incidence in the cultivated varieties and the locations in the different provinces of the Maule Region. It was observed that the Linares province showed the highest levels of the virus in the different varieties: Amity (70%), Meeker (39%) and Heritage (26%), compared to other provinces in the region. These results suggest a high spread of ToRSV through the Maule Region. Nei distance analysis suggests that 14 of the virus isolates coming from the Talca and Linares Provinces would show differences with the ToRSV accessions deposited in the global gene bank (NCBI).
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spelling uncu-93232017-10-12T19:08:42Z Distribution and frequency of tomato ringspot virus (ToRSV) in different varieties of Rubus idaeus in the Maule Region, Chile Distribución y frecuencia de tomato ringspot virus (ToRSV) en diferentes variedades de Rubus idaeus en la Región del Maule, Chile González Silva, Gloria Rossana Concha Espinoza, Cynthia Macarena Valenzuela Bustamante, Myriam Andrea Cordero Alday, Luzmira Cecilia Pico Mendoza, José Newthon Cáceres Ruz, Pablo Alfredo García González, Rolando Tomato ringspot virus Rubus idaeus Región del Maule (Chile) Fitopatología Virus ToRSV Disease Spread Incidence Incidencia de ToRSV Dispersión de ToRSV The raspberry (Rubus idaeus) is one of the most important fruit for production in the Maule Region, Chile. Raspberries are affected by the tomato ringspot virus (ToRSV), which causes decreased yield and deformed fruit. The objective of this work is to study ToRSV spread in different raspberry varieties in the Maule Region, Chile. The virus was detected using the ELISA test and RT-PCR in the Heritage, Meeker, Chilliwack, Amity and Coho varieties. Bayesian analysis determined the relationship between the percentage of ToRSV incidence in the cultivated varieties and the locations in the different provinces of the Maule Region. It was observed that the Linares province showed the highest levels of the virus in the different varieties: Amity (70%), Meeker (39%) and Heritage (26%), compared to other provinces in the region. These results suggest a high spread of ToRSV through the Maule Region. Nei distance analysis suggests that 14 of the virus isolates coming from the Talca and Linares Provinces would show differences with the ToRSV accessions deposited in the global gene bank (NCBI). La frambuesa (Rubus idaeus) es un frutal menor de gran importancia productiva en Chile. Una de las enfermedades que lo afectan es tomato ringspot virus (ToRSV). Este estudio provee información sobre la dispersión de ToRSV en diferentes variedades de frambueso en la Región del Maule. El virus fue detectado mediante el uso de la prueba ELISA y RT-PCR en las variedades Heritage, Meeker, Chilliwack, Amity y Coho. Mediante un análisis Bayesiano se determinó la relación entre porcentaje de incidencia de ToRSV en las variedades cultivadas y las localidades, de las distintas Provincias de la Región. Se observó que la provincia de Linares presentó la más alta incidencia viral en Amity (70%), Meeker (39%) y Heritage (26%), con respecto a otras provincias de la Región. Estos resultados sugieren que existe una alta dispersión de ToRSV a través de la Región del Maule. El análisis de distancia de Nei propone que los 14 aislados del virus provenientes desde las Provincias de Talca y Linares, mostrarían diferencias con las accesiones de ToRSV depositadas en el banco de genes mundial (NCBI). Fil: González Silva, Gloria Rossana. Universidad Católica del Maule. Facultad de Ciencias Agrarias y Forestales Fil: Concha Espinoza, Cynthia Macarena. Universidad Católica del Maule. Facultad de Ciencias Agrarias y Forestales Fil: Valenzuela Bustamante, Myriam Andrea. Universidad Católica del Maule. Facultad de Ciencias Agrarias y Forestales Fil: Cordero Alday, Luzmira Cecilia. Comisión Nacional de Investigación Científica y Tecnológica (CONICYT), Centro de Estudios en Alimentos Procesados ​​(CEAP), Regional, Gore Maule, Talca, Chile. Fil: Pico Mendoza, José Newthon. Universidad Técnica de Manabí. Facultad de Ingeniería Agrícola (Lodana, Manabí, Ecuador) Fil: Cáceres Ruz, Pablo Alfredo. Universidad Católica del Maule. Facultad de Ciencias Agrarias y Forestales Fil: García González, Rolando. Universidad Católica del Maule. Facultad de Ciencias Agrarias y Forestales Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias 78 Revista de la Facultad de Ciencias Agrarias Vol. 49, no. 1 Revista de la Facultad de Ciencias Agrarias, Vol. 49, no. 1 2017-03-01 eng Inglés http://bdigital.uncu.edu.ar/8856 info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar/ article info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias http://bdigital.uncu.edu.ar/9323
spellingShingle Tomato ringspot virus
Rubus idaeus
Región del Maule (Chile)
Fitopatología
Virus
ToRSV
Disease
Spread
Incidence
Incidencia de ToRSV
Dispersión de ToRSV
González Silva, Gloria Rossana
Concha Espinoza, Cynthia Macarena
Valenzuela Bustamante, Myriam Andrea
Cordero Alday, Luzmira Cecilia
Pico Mendoza, José Newthon
Cáceres Ruz, Pablo Alfredo
García González, Rolando
Distribution and frequency of tomato ringspot virus (ToRSV) in different varieties of Rubus idaeus in the Maule Region, Chile
title Distribution and frequency of tomato ringspot virus (ToRSV) in different varieties of Rubus idaeus in the Maule Region, Chile
title_full Distribution and frequency of tomato ringspot virus (ToRSV) in different varieties of Rubus idaeus in the Maule Region, Chile
title_fullStr Distribution and frequency of tomato ringspot virus (ToRSV) in different varieties of Rubus idaeus in the Maule Region, Chile
title_full_unstemmed Distribution and frequency of tomato ringspot virus (ToRSV) in different varieties of Rubus idaeus in the Maule Region, Chile
title_short Distribution and frequency of tomato ringspot virus (ToRSV) in different varieties of Rubus idaeus in the Maule Region, Chile
title_sort distribution and frequency of tomato ringspot virus (torsv) in different varieties of rubus idaeus in the maule region, chile
topic Tomato ringspot virus
Rubus idaeus
Región del Maule (Chile)
Fitopatología
Virus
ToRSV
Disease
Spread
Incidence
Incidencia de ToRSV
Dispersión de ToRSV
url http://bdigital.uncu.edu.ar/9323