Biblioteca Digital de VanguardiaGenetic diversity among alfalfa genotypes (Medicago sativa L.) of non-dormant cultivars using SSR markers and agronomic traits
The aim of this study was to assess genetic diversity among 40 alfalfa (Medicago sativa L.) genotypes of different non-dormant (FD=8) cultivars. Biomass yield, regrowth speed and reaction to spring black stem, lepto leaf spot, and rust were evaluated. Analyses of variances were performed using a mix...
| Autores principales: | , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias
2013
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| Acceso en línea: | http://bdigital.uncu.edu.ar/6112 |
| _version_ | 1782341361182179328 |
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| author | Grandón, Nancy G. Alarcón, Yanina Moreno, María V. Arolfo, Valeria Orodizzi, Ariel Basigalup, Daniel H. Gieco, Jorge O. Bruno, Cecilia |
| author_facet | Grandón, Nancy G. Alarcón, Yanina Moreno, María V. Arolfo, Valeria Orodizzi, Ariel Basigalup, Daniel H. Gieco, Jorge O. Bruno, Cecilia |
| author_sort | Grandón, Nancy G. |
| collection | Repositorio |
| description | The aim of this study was to assess
genetic diversity among 40 alfalfa (Medicago
sativa L.) genotypes of different non-dormant
(FD=8) cultivars. Biomass yield, regrowth
speed and reaction to spring black stem, lepto
leaf spot, and rust were evaluated. Analyses of
variances were performed using a mixed model
to examine the agronomic variation among
individuals. A principal component analysis on
standardized agronomic data was performed.
Agronomic data were also used to calculate
Gower's distance and UPGMA algorithm. For
the molecular analysis, six SSR markers were
evaluated and 84 alleles were identified. The
genetic distance was estimated using standard
Nei's distance. Average standard genetic
diversity was 0.843, indicating a high degree
of variability among genotypes. Finally, a
generalized procrustes analysis was performed
to calculate the correlation between molecular
and agronomic distance, indicating a 65.4%
of consensus. This value is likely related to
the low number of individuals included in the
study, which might have underestimated the
real phenotypic variability among genotypes.
Despite the low number of individuals and
SSR markers analyzed, this study provides a
baseline for future diversity studies to identify
genetically distant alfalfa individuals or cultivars. |
| format | Artículo |
| id | uncu-6112 |
| institution | Biblioteca Digital - UNCUYO |
| language | Inglés |
| publishDate | 2013 |
| publisher | Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias
|
| record_format | ojs |
| spelling | uncu-61122014-10-22T11:25:13Z Genetic diversity among alfalfa genotypes (Medicago sativa L.) of non-dormant cultivars using SSR markers and agronomic traits Análisis de la diversidad genética en genotipos de alfalfa (Medicago sativa L.) sin dormición, mediante el uso de marcadores SSR y caracteres agronómicos Grandón, Nancy G. Alarcón, Yanina Moreno, María V. Arolfo, Valeria Orodizzi, Ariel Basigalup, Daniel H. Gieco, Jorge O. Bruno, Cecilia Diversidad genética como recurso Genotipos Medicago sativa Autotetraploide Fitomejoramiento Genetic diversity as resource Genotypes Autotetraploidy Plant breeding Alfalfa Análisis de clusters Análisis de componentes principales Análisis de Procrustes Generalizado Mejoramiento vegetal Microsatellite marker Cluster analysis Principal components analysis Generalizes procrustes analysis The aim of this study was to assess genetic diversity among 40 alfalfa (Medicago sativa L.) genotypes of different non-dormant (FD=8) cultivars. Biomass yield, regrowth speed and reaction to spring black stem, lepto leaf spot, and rust were evaluated. Analyses of variances were performed using a mixed model to examine the agronomic variation among individuals. A principal component analysis on standardized agronomic data was performed. Agronomic data were also used to calculate Gower's distance and UPGMA algorithm. For the molecular analysis, six SSR markers were evaluated and 84 alleles were identified. The genetic distance was estimated using standard Nei's distance. Average standard genetic diversity was 0.843, indicating a high degree of variability among genotypes. Finally, a generalized procrustes analysis was performed to calculate the correlation between molecular and agronomic distance, indicating a 65.4% of consensus. This value is likely related to the low number of individuals included in the study, which might have underestimated the real phenotypic variability among genotypes. Despite the low number of individuals and SSR markers analyzed, this study provides a baseline for future diversity studies to identify genetically distant alfalfa individuals or cultivars. El objetivo de este trabajo consistió en determinar la diversidad genética en 40 individuos de alfalfa (Medicago sativa L.) de cinco cultivares de grado de reposo 8. Se evaluaron a campo la producción de forraje, la velocidad de rebrote y el comportamiento frente a tallo negro de primavera, mancha ocular y roya. El análisis de la varianza se realizó mediante un modelo lineal mixto para determinar la variación agronómica entre los individuos. El análisis de componentes principales se realizó a partir de los datos agronómicos estandarizados. El dendrograma agronómico se construyó a partir del índice de Gower y el agrupamiento UPGMA. Para la caracterización molecular se analizaron seis marcadores SSR, con los cuales se identificaron un total de 84 alelos. Las distancias genéticas se calcularon con el índice de Nei estándar. La diversidad genética promedio fue de 0,843, indicando una alta variabilidad entre los individuos evaluados. Por último, el análisis de procrustes generalizado detectó un 65,4% de consenso entre el ordenamiento agronómico y el molecular. Este porcentaje probablemente se relacione con el bajo número de individuos y marcadores SSR analizados; sin embargo, este estudio provee una línea de base para estudios futuros de diversidad que permitirán identificar individuos o cultivares genéticamente distantes. Fil: Grandón, Nancy G.. Instituto Nacional de Tecnología Agropecuaria (Argentina). Estación Experimental Agropecuaria Manfredi. Fil: Alarcón, Yanina. University of Georgia Fil: Moreno, María V.. Instituto Nacional de Tecnología Agropecuaria (Argentina). Estación Experimental Agropecuaria Manfredi. Fil: Arolfo, Valeria. Instituto Nacional de Tecnología Agropecuaria (Argentina). Estación Experimental Agropecuaria Manfredi. Fil: Orodizzi, Ariel. Instituto Nacional de Tecnología Agropecuaria (Argentina). Estación Experimental Agropecuaria Manfredi. Fil: Basigalup, Daniel H.. Instituto Nacional de Tecnología Agropecuaria (Argentina). Estación Experimental Agropecuaria Manfredi. Fil: Gieco, Jorge O.. Instituto Nacional de Tecnología Agropecuaria (Argentina). Estación Experimental Agropecuaria Manfredi. Fil: Bruno, Cecilia. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Cátedra de Estadística y Biometría. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias 78 Revista de la Facultad de Ciencias Agrarias Vol. 45, no. 2 Revista de la Facultad de Ciencias Agrarias, Vol. 45, no. 2 2013-12-02 eng Inglés http://bdigital.uncu.edu.ar/6005 info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar/ article info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias http://bdigital.uncu.edu.ar/6112 |
| spellingShingle | Diversidad genética como recurso Genotipos Medicago sativa Autotetraploide Fitomejoramiento Genetic diversity as resource Genotypes Autotetraploidy Plant breeding Alfalfa Análisis de clusters Análisis de componentes principales Análisis de Procrustes Generalizado Mejoramiento vegetal Microsatellite marker Cluster analysis Principal components analysis Generalizes procrustes analysis Grandón, Nancy G. Alarcón, Yanina Moreno, María V. Arolfo, Valeria Orodizzi, Ariel Basigalup, Daniel H. Gieco, Jorge O. Bruno, Cecilia Genetic diversity among alfalfa genotypes (Medicago sativa L.) of non-dormant cultivars using SSR markers and agronomic traits |
| title | Genetic diversity among alfalfa genotypes (Medicago sativa L.) of non-dormant cultivars using SSR markers and agronomic traits
|
| title_full | Genetic diversity among alfalfa genotypes (Medicago sativa L.) of non-dormant cultivars using SSR markers and agronomic traits
|
| title_fullStr | Genetic diversity among alfalfa genotypes (Medicago sativa L.) of non-dormant cultivars using SSR markers and agronomic traits
|
| title_full_unstemmed | Genetic diversity among alfalfa genotypes (Medicago sativa L.) of non-dormant cultivars using SSR markers and agronomic traits
|
| title_short | Genetic diversity among alfalfa genotypes (Medicago sativa L.) of non-dormant cultivars using SSR markers and agronomic traits
|
| title_sort | genetic diversity among alfalfa genotypes (medicago sativa l.) of non-dormant cultivars using ssr markers and agronomic traits |
| topic | Diversidad genética como recurso Genotipos Medicago sativa Autotetraploide Fitomejoramiento Genetic diversity as resource Genotypes Autotetraploidy Plant breeding Alfalfa Análisis de clusters Análisis de componentes principales Análisis de Procrustes Generalizado Mejoramiento vegetal Microsatellite marker Cluster analysis Principal components analysis Generalizes procrustes analysis |
| url | http://bdigital.uncu.edu.ar/6112 |