| Sumario: | The aim of this study was to assess
genetic diversity among 40 alfalfa (Medicago
sativa L.) genotypes of different non-dormant
(FD=8) cultivars. Biomass yield, regrowth
speed and reaction to spring black stem, lepto
leaf spot, and rust were evaluated. Analyses of
variances were performed using a mixed model
to examine the agronomic variation among
individuals. A principal component analysis on
standardized agronomic data was performed.
Agronomic data were also used to calculate
Gower's distance and UPGMA algorithm. For
the molecular analysis, six SSR markers were
evaluated and 84 alleles were identified. The
genetic distance was estimated using standard
Nei's distance. Average standard genetic
diversity was 0.843, indicating a high degree
of variability among genotypes. Finally, a
generalized procrustes analysis was performed
to calculate the correlation between molecular
and agronomic distance, indicating a 65.4%
of consensus. This value is likely related to
the low number of individuals included in the
study, which might have underestimated the
real phenotypic variability among genotypes.
Despite the low number of individuals and
SSR markers analyzed, this study provides a
baseline for future diversity studies to identify
genetically distant alfalfa individuals or cultivars.
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