Biblioteca Digital de VanguardiaEffect of colchicine and amiprophos-methyl on the production of in vitro doubled haploid onion plants and correlation assessment between ploidy level and stomatal size
Doubled haploid onion (Allium cepa L.) plants allow the production of completely homozygous lines for a later production of hybrids. The haploid plants are normally produced using in vitro gynogenesis. The obtained haploid plantlets must be treated with different agents for doubling chromosomes. It...
| Autores principales: | , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias
2013
|
| Materias: | |
| Acceso en línea: | http://bdigital.uncu.edu.ar/6107 |
| _version_ | 1782341359875653632 |
|---|---|
| author | Foschi, María Laura Martínez, Liliana Ponce, María T. Galmarini, Claudio Bohanec, Borut |
| author_facet | Foschi, María Laura Martínez, Liliana Ponce, María T. Galmarini, Claudio Bohanec, Borut |
| author_sort | Foschi, María Laura |
| collection | Repositorio |
| description | Doubled haploid onion (Allium cepa L.)
plants allow the production of completely
homozygous lines for a later production of hybrids.
The haploid plants are normally produced using in
vitro gynogenesis. The obtained haploid plantlets
must be treated with different agents for doubling
chromosomes. It is necessary to adjust the
concentration and the length of treatment of the
doubling agent. In this case, the effect of 250 and
500 mg.L-1 colchicine and 15.2; 30 and 60 mg.L- 1
amiprophos-methyl during 24 and 48 h was
assessed over the rate of onion haploid plantlets
chromosome doubling. The best duplication
treatment was 250 mg.L-1 colchicine for 48 h,
which yielded 100% of doubled haploid plants. On
the other hand, a positive correlation resulted from
the ploidy level and stomatal size, and a negative
correlation between the level of ploidy and
stomatal density. Significant differences between
the stomatal length, width and density in haploid
and doubled haploid plantlets were observed.
An economical and quick method to test ploidy
level in onion plantlets is proposed through the
measurement of stomatal size and density. |
| format | Artículo |
| id | uncu-6107 |
| institution | Biblioteca Digital - UNCUYO |
| language | Inglés |
| publishDate | 2013 |
| publisher | Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias
|
| record_format | ojs |
| spelling | uncu-61072014-10-20T13:45:48Z Effect of colchicine and amiprophos-methyl on the production of in vitro doubled haploid onion plants and correlation assessment between ploidy level and stomatal size Efecto de la colchicina y del amiprofos-metil en la producción in vitro de plantas dihaploides de cebolla y determinación de la correlación entre el nivel de ploidía y tamaño de los estomas Foschi, María Laura Martínez, Liliana Ponce, María T. Galmarini, Claudio Bohanec, Borut Colchicina Cebolla Ploidía Allium cepa Duplicación de genes Ginogénesis Híbridos Colchicine Onions Ploidy Gynogenesis Hybrids Plantas dihaploides de cebolla Doubled haploid onion (Allium cepa L.) plants allow the production of completely homozygous lines for a later production of hybrids. The haploid plants are normally produced using in vitro gynogenesis. The obtained haploid plantlets must be treated with different agents for doubling chromosomes. It is necessary to adjust the concentration and the length of treatment of the doubling agent. In this case, the effect of 250 and 500 mg.L-1 colchicine and 15.2; 30 and 60 mg.L- 1 amiprophos-methyl during 24 and 48 h was assessed over the rate of onion haploid plantlets chromosome doubling. The best duplication treatment was 250 mg.L-1 colchicine for 48 h, which yielded 100% of doubled haploid plants. On the other hand, a positive correlation resulted from the ploidy level and stomatal size, and a negative correlation between the level of ploidy and stomatal density. Significant differences between the stomatal length, width and density in haploid and doubled haploid plantlets were observed. An economical and quick method to test ploidy level in onion plantlets is proposed through the measurement of stomatal size and density. La producción de plantas dihaploides de cebolla (Allium cepa L.), permite obtener líneas completamente homocigotas para luego producir híbridos. A través de la ginogénesis in vitro se ha logrado producir plantas haploides, las cuales deben ser sometidas a tratamientos de duplicación de sus cromosomas, siendo necesario ajustar la concentración del agente duplicador y la duración del tratamiento. En este trabajo se evaluó el efecto de la aplicación de 250 y 500 mg.L-1 de colchicina y 15,2; 30 y 60 mg.L-1 de amiprofos-metil durante 24 y 48 h, sobre la tasa de duplicación de cromosomas en plántulas haploides de cebolla. El mejor tratamiento de duplicación fue 250 mg.L- 1 de colchicina, durante 48 h, logrando un 100% de plantas dihaploides. Por otra parte, se obtuvo una correlación positiva entre el nivel de ploidía y el tamaño de estomas y una correlación negativa entre el nivel de ploidía y la densidad estomática, registrándose diferencias significativas entre los valores promedio de largo, ancho y densidad de estomas para plantas haploides y dihaploides. Se propone un método económico y rápido para verificar el nivel de ploidía de las plántulas de cebolla mediante la medición del tamaño y densidad estomática. Fil: Foschi, María Laura. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias Fil: Martínez, Liliana. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias Fil: Ponce, María T.. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias Fil: Galmarini, Claudio. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias Fil: Bohanec, Borut. University of Ljubljana, Jamnikarjeva. Biotechnical Faculty. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias 78 Revista de la Facultad de Ciencias Agrarias Vol. 45, no. 2 Revista de la Facultad de Ciencias Agrarias, Vol. 45, no. 2 2013-12-02 eng Inglés http://bdigital.uncu.edu.ar/6005 info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar/ article info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias http://bdigital.uncu.edu.ar/6107 |
| spellingShingle | Colchicina Cebolla Ploidía Allium cepa Duplicación de genes Ginogénesis Híbridos Colchicine Onions Ploidy Gynogenesis Hybrids Plantas dihaploides de cebolla Foschi, María Laura Martínez, Liliana Ponce, María T. Galmarini, Claudio Bohanec, Borut Effect of colchicine and amiprophos-methyl on the production of in vitro doubled haploid onion plants and correlation assessment between ploidy level and stomatal size |
| title | Effect of colchicine and amiprophos-methyl on the production of in vitro doubled haploid onion plants and correlation assessment between ploidy level and stomatal size
|
| title_full | Effect of colchicine and amiprophos-methyl on the production of in vitro doubled haploid onion plants and correlation assessment between ploidy level and stomatal size
|
| title_fullStr | Effect of colchicine and amiprophos-methyl on the production of in vitro doubled haploid onion plants and correlation assessment between ploidy level and stomatal size
|
| title_full_unstemmed | Effect of colchicine and amiprophos-methyl on the production of in vitro doubled haploid onion plants and correlation assessment between ploidy level and stomatal size
|
| title_short | Effect of colchicine and amiprophos-methyl on the production of in vitro doubled haploid onion plants and correlation assessment between ploidy level and stomatal size
|
| title_sort | effect of colchicine and amiprophos-methyl on the production of in vitro doubled haploid onion plants and correlation assessment between ploidy level and stomatal size |
| topic | Colchicina Cebolla Ploidía Allium cepa Duplicación de genes Ginogénesis Híbridos Colchicine Onions Ploidy Gynogenesis Hybrids Plantas dihaploides de cebolla |
| url | http://bdigital.uncu.edu.ar/6107 |