Biblioteca Digital de VanguardiaGamma irradiation on Alstroemeria aurea G. in vitro rhizomes: : an approach to the appropriate dosage for breeding purposes
Gamma irradiation has been widely used as a breeding technique to obtain new cultivars in ornamental species such as Alstroemeria, where several cultivars have been obtained through rhizome radiation. The optimum dosage for an appropriate induction of mutation must be considered for breeding purpose...
| Autores principales: | , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias
2012
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| Materias: | |
| Acceso en línea: | http://bdigital.uncu.edu.ar/4523 |
| _version_ | 1782341025445969920 |
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| author | Aros, Danilo Valdés, Santiago Olate, Eduardo Infante, Rodrigo |
| author_facet | Aros, Danilo Valdés, Santiago Olate, Eduardo Infante, Rodrigo |
| author_sort | Aros, Danilo |
| collection | Repositorio |
| description | Gamma irradiation has been widely used
as a breeding technique to obtain new cultivars
in ornamental species such as Alstroemeria,
where several cultivars have been obtained
through rhizome radiation. The optimum dosage
for an appropriate induction of mutation must be
considered for breeding purposes and it depends
mainly on plant susceptibility. Thus in this study
in vitro cultured rhizomes of Alstroemeria aurea
were irradiated with a gamma source using
different dosages to evaluate the direct effect
produced. Damage and number of rhizome
sprouting were observed and recorded during
61 days after irradiation. At the end of this period,
rhizomes were weighted and mortality was
evaluated. Both mortality and weight increased
depending on dosage. All irradiated rhizomes
showed early sprouting in comparison with
control (0 Gy) and no significant difference in final
number of shoots after 61 days among irradiated
treatments was observed. Bleaching and necrosis
was observed in all irradiated rhizomes and
was more evident at higher doses. LD50 was
established at about 40 Gy and the optimum
dosage to induce mutation was suggested
between 2.5 and 5 Gy, when the growth was
reduced in 50%, and probably this dosage could
be used for breeding purposes.
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| format | Artículo |
| id | uncu-4523 |
| institution | Biblioteca Digital - UNCUYO |
| language | Inglés |
| publishDate | 2012 |
| publisher | Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias
|
| record_format | ojs |
| spelling | uncu-45232012-07-03T15:19:01Z Gamma irradiation on Alstroemeria aurea G. in vitro rhizomes: : an approach to the appropriate dosage for breeding purposes Irradiación de rizomas in vitro de Alstroemeria aurea G. con rayos gamma: : una aproximación a la dosis apropiada para utilizar en mejoramiento genético Aros, Danilo Valdés, Santiago Olate, Eduardo Infante, Rodrigo Chile Mutación Brotación Mejoramiento genético Irradiación gamma Mutation Sprouting Breeding Radio susceptibilidad Gamma irradiation has been widely used as a breeding technique to obtain new cultivars in ornamental species such as Alstroemeria, where several cultivars have been obtained through rhizome radiation. The optimum dosage for an appropriate induction of mutation must be considered for breeding purposes and it depends mainly on plant susceptibility. Thus in this study in vitro cultured rhizomes of Alstroemeria aurea were irradiated with a gamma source using different dosages to evaluate the direct effect produced. Damage and number of rhizome sprouting were observed and recorded during 61 days after irradiation. At the end of this period, rhizomes were weighted and mortality was evaluated. Both mortality and weight increased depending on dosage. All irradiated rhizomes showed early sprouting in comparison with control (0 Gy) and no significant difference in final number of shoots after 61 days among irradiated treatments was observed. Bleaching and necrosis was observed in all irradiated rhizomes and was more evident at higher doses. LD50 was established at about 40 Gy and the optimum dosage to induce mutation was suggested between 2.5 and 5 Gy, when the growth was reduced in 50%, and probably this dosage could be used for breeding purposes. La irradiación con rayos gamma ha sido ampliamente utilizada como herramienta en mejoramiento genético para obtener nuevos cultivares de especies ornamentales tales como Alstroemeria, en que varios cultivares se han obtenido mediante la irradiación de rizomas. La dosis óptima para una apropiada inducción de mutación debe ser considerada para fines de mejoramiento genético y depende principalmente de la susceptibilidad de la planta. En este estudio, rizomas de Alstroemeria aurea cultivados in vitro fueron irradiados con una fuente de rayos gamma utilizando diferentes dosis para evaluar el efecto directo producido. El daño y número de rizomas en brotación fueron observados y registrados durante 61 días después de la irradiación. Al final de este periodo, los rizomas fueron pesados y la mortalidad fue evaluada. Tanto la mortalidad como el peso aumentaron dependiendo de la dosis. Todos los rizomas irradiados mostraron una brotación anticipada en comparación con el control (0 Gy) y no se observaron diferencias significativas en el número final de brotes después de los 61 días entre los diferentes tratamientos. Se observó blanqueamiento y necrosis en todos los rizomas irradiados, siendo más evidente a altas dosis. El DL50 fue establecido alrededor de 40 Gy y la dosis óptima para inducir mutación se sugiere entre 2,5 y 5 Gy, cuando el crecimiento se redujo en un 50%, y probablemente esta dosis pueda ser utilizada con fines de mejoramiento genético. Fil: Aros, Danilo. Universidad de Chile. Facultad de Ciencias Agronómicas. Fil: Valdés, Santiago. Universidad de Chile. Facultad de Ciencias Agronómicas. Fil: Olate, Eduardo. Fil: Infante, Rodrigo. Universidad de Chile. Facultad de Ciencias Agronómicas. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias 78 Revista de la Facultad de Ciencias Agrarias Vol. 44, no. 1 Revista de la Facultad de Ciencias Agrarias, Vol. 44, no. 1 2012-04-02 eng Inglés http://bdigital.uncu.edu.ar/4482 info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar/ article info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias http://bdigital.uncu.edu.ar/4523 |
| spellingShingle | Chile Mutación Brotación Mejoramiento genético Irradiación gamma Mutation Sprouting Breeding Radio susceptibilidad Aros, Danilo Valdés, Santiago Olate, Eduardo Infante, Rodrigo Gamma irradiation on Alstroemeria aurea G. in vitro rhizomes: : an approach to the appropriate dosage for breeding purposes |
| title | Gamma irradiation on Alstroemeria aurea G. in vitro rhizomes:
: an approach to the appropriate dosage for breeding purposes |
| title_full | Gamma irradiation on Alstroemeria aurea G. in vitro rhizomes:
: an approach to the appropriate dosage for breeding purposes |
| title_fullStr | Gamma irradiation on Alstroemeria aurea G. in vitro rhizomes:
: an approach to the appropriate dosage for breeding purposes |
| title_full_unstemmed | Gamma irradiation on Alstroemeria aurea G. in vitro rhizomes:
: an approach to the appropriate dosage for breeding purposes |
| title_short | Gamma irradiation on Alstroemeria aurea G. in vitro rhizomes:
: an approach to the appropriate dosage for breeding purposes |
| title_sort | gamma irradiation on alstroemeria aurea g. in vitro rhizomes:
: an approach to the appropriate dosage for breeding purposes |
| topic | Chile Mutación Brotación Mejoramiento genético Irradiación gamma Mutation Sprouting Breeding Radio susceptibilidad |
| url | http://bdigital.uncu.edu.ar/4523 |