Biblioteca Digital de VanguardiaEffect of culture medium on morphogenic processes in vitro in Cinchona officinalis L.
This paper describes the effect of the various plant growth regulators of the culture medium on morphogenic processes in vitro in Cinchona officinalis L, a highly vulnerable species from southern Ecuador. To do this, different concentrations of NaOCl were used in combination with different immersion...
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias
2019
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| Acceso en línea: | http://bdigital.uncu.edu.ar/13400 |
| _version_ | 1782342940960489472 |
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| author | Moreno Serrano, José Antonio Pérez Ruíz, César Moreno Fierro, Ivonne Moreno Fierro, Jorge |
| author_facet | Moreno Serrano, José Antonio Pérez Ruíz, César Moreno Fierro, Ivonne Moreno Fierro, Jorge |
| author_sort | Moreno Serrano, José Antonio |
| collection | Repositorio |
| description | This paper describes the effect of the various plant growth regulators of the culture
medium on morphogenic processes in vitro in Cinchona officinalis L, a highly vulnerable
species from southern Ecuador. To do this, different concentrations of NaOCl were used in
combination with different immersion times for seed disinfection; for seed germination
in vitro GA3 was added to the MS basal culture medium in different concentrations, and
for morphogenic processes in vitro, different concentrations of auxins and cytokinins
were combined. The decrease in the contamination rate was with high concentrations
of NaOCl and an increase in the germination rate in 45 days with the addition of 1.0 mg
L-1 GA3 to the culture medium the hormonal combination of 0.5 mg L-1 NAA + 2.5 mg L-1
BAP showed a high rate of shoot proliferation and with 1.0 mg L-1 NAA a high number
of roots was obtained. In the callogenesis phase, the best results were obtained with 1.0
mg L-1 2,4-D + 0.5 mg L-1 BAP for callus proliferation. In vitro propagation protocols were
generated in Cinchona officinalis L, for the preservation and conservation of the species. |
| format | Artículo |
| id | uncu-13400 |
| institution | Biblioteca Digital - UNCUYO |
| language | Inglés |
| publishDate | 2019 |
| publisher | Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias
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| record_format | ojs |
| spelling | uncu-134002019-10-02T17:29:03Z Effect of culture medium on morphogenic processes in vitro in Cinchona officinalis L. Efecto del medio de cultivo en los procesos morfogénicos in vitro en Cinchona officinalis L. Moreno Serrano, José Antonio Pérez Ruíz, César Moreno Fierro, Ivonne Moreno Fierro, Jorge Germinación Cultivo in vitro Cinchona officinalis Callogénesis Organogénesis Sustancias de crecimiento vegetal Plant growth Germination Organogenesis Callogenesis Reguladores del crecimiento This paper describes the effect of the various plant growth regulators of the culture medium on morphogenic processes in vitro in Cinchona officinalis L, a highly vulnerable species from southern Ecuador. To do this, different concentrations of NaOCl were used in combination with different immersion times for seed disinfection; for seed germination in vitro GA3 was added to the MS basal culture medium in different concentrations, and for morphogenic processes in vitro, different concentrations of auxins and cytokinins were combined. The decrease in the contamination rate was with high concentrations of NaOCl and an increase in the germination rate in 45 days with the addition of 1.0 mg L-1 GA3 to the culture medium the hormonal combination of 0.5 mg L-1 NAA + 2.5 mg L-1 BAP showed a high rate of shoot proliferation and with 1.0 mg L-1 NAA a high number of roots was obtained. In the callogenesis phase, the best results were obtained with 1.0 mg L-1 2,4-D + 0.5 mg L-1 BAP for callus proliferation. In vitro propagation protocols were generated in Cinchona officinalis L, for the preservation and conservation of the species. Este trabajo describe el efecto de los diversos reguladores de crecimiento del medio de cultivo en los procesos morfogénicos in vitro en Cinchona officinalis L, una especie altamente vulnerable del sur de Ecuador. Para ello, se utilizó diferentes concentraciones de NaOCl en combinación con distintos tiempos de inmersión para la desinfección de semillas; para la germinación in vitro de semillas se agregó AG3 al medio de cultivo MS basal en diferentes concentraciones, y para los procesos morfogénicos in vitro se combinaron diferentes concentraciones de auxinas y citocininas. Se obtuvo la disminución de la tasa de contaminación con altas concentraciones de NaOCl y aumento de la tasa de germinación en 45 días con la adición de 1,0 mg L-1 AG3 al medio de cultivo; la combinación hormonal de 0,5 mg L-1 ANA + 2,5 mg L-1 BAP mostró una alta tasa de proliferación de brotes y con 1,0 mg L-1 ANA se logró obtener un alto número de raíces/explante. En la fase de callogénesis, los mejores resultados se obtuvieron con 1,0 mg L-1 2,4-D + 0,5 mg L-1 BAP para proliferación de callo. Se generaron protocolos de propagación in vitro en Cinchona officinalis L, con fines de preservación y conservación de la especie. Fil: Moreno Serrano, José Antonio. Universidad Politécnica de Madrid. Departamento de Biología Vegetal Fil: Pérez Ruíz, César. Universidad Politécnica de Madrid. Departamento de Biología Vegetal Fil: Moreno Fierro, Ivonne. Universidad Politécnica de Madrid. Departamento de Biología Vegetal Fil: Moreno Fierro, Jorge. University of Bedfordshire (Inglaterra) Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias 78 Revista de la Facultad de Ciencias Agrarias Vol. 51, no. 1 Revista de la Facultad de Ciencias Agrarias, Vol. 51, no. 1 2019-02-01 eng Inglés http://bdigital.uncu.edu.ar/12688 info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar/ article info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias http://bdigital.uncu.edu.ar/13400 |
| spellingShingle | Germinación Cultivo in vitro Cinchona officinalis Callogénesis Organogénesis Sustancias de crecimiento vegetal Plant growth Germination Organogenesis Callogenesis Reguladores del crecimiento Moreno Serrano, José Antonio Pérez Ruíz, César Moreno Fierro, Ivonne Moreno Fierro, Jorge Effect of culture medium on morphogenic processes in vitro in Cinchona officinalis L. |
| title | Effect of culture medium on morphogenic processes in vitro in Cinchona officinalis L.
|
| title_full | Effect of culture medium on morphogenic processes in vitro in Cinchona officinalis L.
|
| title_fullStr | Effect of culture medium on morphogenic processes in vitro in Cinchona officinalis L.
|
| title_full_unstemmed | Effect of culture medium on morphogenic processes in vitro in Cinchona officinalis L.
|
| title_short | Effect of culture medium on morphogenic processes in vitro in Cinchona officinalis L.
|
| title_sort | effect of culture medium on morphogenic processes in vitro in cinchona officinalis l. |
| topic | Germinación Cultivo in vitro Cinchona officinalis Callogénesis Organogénesis Sustancias de crecimiento vegetal Plant growth Germination Organogenesis Callogenesis Reguladores del crecimiento |
| url | http://bdigital.uncu.edu.ar/13400 |