| Sumario: | This paper describes the effect of the various plant growth regulators of the culture
medium on morphogenic processes in vitro in Cinchona officinalis L, a highly vulnerable
species from southern Ecuador. To do this, different concentrations of NaOCl were used in
combination with different immersion times for seed disinfection; for seed germination
in vitro GA3 was added to the MS basal culture medium in different concentrations, and
for morphogenic processes in vitro, different concentrations of auxins and cytokinins
were combined. The decrease in the contamination rate was with high concentrations
of NaOCl and an increase in the germination rate in 45 days with the addition of 1.0 mg
L-1 GA3 to the culture medium the hormonal combination of 0.5 mg L-1 NAA + 2.5 mg L-1
BAP showed a high rate of shoot proliferation and with 1.0 mg L-1 NAA a high number
of roots was obtained. In the callogenesis phase, the best results were obtained with 1.0
mg L-1 2,4-D + 0.5 mg L-1 BAP for callus proliferation. In vitro propagation protocols were
generated in Cinchona officinalis L, for the preservation and conservation of the species.
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