Biblioteca Digital de VanguardiaAnalysis of genetic variability in vitro regenerated buffelgrass plants through ISSR molecular markers
Genetic variability can be generated through in vitro culture via somaclonal variation. This tool can be potentially useful in a breeding program involving apomictic buffelgrass genotypes. The aim of this work was to evaluate inter simple sequence repeats (ISSR) as molecular markers to detect geneti...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias
2018
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| Acceso en línea: | http://bdigital.uncu.edu.ar/11586 |
| _version_ | 1782342536306622464 |
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| author | Carloni, Edgardo López Colomba, Eliana Ribotta, Andrea Quiroga, Mariana Tommasino, Exequiel Griffa, Sabrina Grunberg, Karina |
| author_facet | Carloni, Edgardo López Colomba, Eliana Ribotta, Andrea Quiroga, Mariana Tommasino, Exequiel Griffa, Sabrina Grunberg, Karina |
| author_sort | Carloni, Edgardo |
| collection | Repositorio |
| description | Genetic variability can be generated through in vitro culture via somaclonal variation.
This tool can be potentially useful in a breeding program involving apomictic
buffelgrass genotypes. The aim of this work was to evaluate inter simple sequence
repeats (ISSR) as molecular markers to detect genetic variation in in vitro buffelgrass
regenerated plants. Six plants regenerated from in vitro anther culture, via somatic
embryogenesis were used, as well as the anther donor genotype (RN 51) as control.
Of a total of 26 ISSR primers tested, 22 amplified, detecting 12% polymorphism with
a divergence between 5 and 24% from RN 51. Amplification products were observed
with the primers containing di-, tri- or tetra-nucleotide sequences, with or without additional
nucleotides at the 3′ end. The most informative primers were those containing the
repetitive sequences GACAn, AGn or GAn. Moreover, the regenerants transplanted at field
conditions differed in morphological characteristics among them and with respect to
RN 51. This study confirms that ISSR are useful to identify genetic variability in in vitro
regenerated buffelgrass plants. |
| format | Artículo |
| id | uncu-11586 |
| institution | Biblioteca Digital - UNCUYO |
| language | Inglés |
| publishDate | 2018 |
| publisher | Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias
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| record_format | ojs |
| spelling | uncu-115862019-02-19T17:03:16Z Analysis of genetic variability in vitro regenerated buffelgrass plants through ISSR molecular markers Análisis de variabilidad genética en plantas regeneradas in vitro de buffelgrass mediante marcadores moleculares ISSR Carloni, Edgardo López Colomba, Eliana Ribotta, Andrea Quiroga, Mariana Tommasino, Exequiel Griffa, Sabrina Grunberg, Karina Variación genética Genética vegetal Regeneración in vitro Cenchrus ciliaris Marcadores genéticos Cultivo in vitro Apomixis Embriogénesis somática Variación somaclonal Cultivo de anteras Diversidad genética como recurso Apomixis Somatic embryogenesis Anther culture ISSR Genetic diversity Análisis de variabilidad Buffelgrass Genetic variability can be generated through in vitro culture via somaclonal variation. This tool can be potentially useful in a breeding program involving apomictic buffelgrass genotypes. The aim of this work was to evaluate inter simple sequence repeats (ISSR) as molecular markers to detect genetic variation in in vitro buffelgrass regenerated plants. Six plants regenerated from in vitro anther culture, via somatic embryogenesis were used, as well as the anther donor genotype (RN 51) as control. Of a total of 26 ISSR primers tested, 22 amplified, detecting 12% polymorphism with a divergence between 5 and 24% from RN 51. Amplification products were observed with the primers containing di-, tri- or tetra-nucleotide sequences, with or without additional nucleotides at the 3′ end. The most informative primers were those containing the repetitive sequences GACAn, AGn or GAn. Moreover, the regenerants transplanted at field conditions differed in morphological characteristics among them and with respect to RN 51. This study confirms that ISSR are useful to identify genetic variability in in vitro regenerated buffelgrass plants. El cultivo in vitro permite generar variabilidad genética mediante variación somaclonal. Este fenómeno puede ser potencialmente útil en un programa de mejora en genotipos apomícticos de buffelgrass. El objetivo del presente estudio fue evaluar inter secuencias simples repetidas (ISSR) como marcadores moleculares en la detección de variación genética en plantas regeneradas in vitro de buffelgrass. Se utilizaron seis plantas regeneradas mediante el cultivo in vitro de anteras, vía embriogénesis somática, y el genotipo dador de anteras (NR 51) como control. De un total de 26 cebadores ISSR probados, 22 de ellos amplificaron, detectando un 12% de polimorfismo con una divergencia del 5 al 24% con respecto al material NR 51. Se observaron productos de amplificación con los cebadores que contienen secuencias di-, tri- o tetra-nucleótidos, con o sin nucleótidos adicionales en el extremo 3′. Los cebadores más informativos fueron aquellos que contenían secuencias repetitivas GACAn, AGn o GAn. Conjuntamente, las plantas trasplantadas a campo manifestaron características morfológicas diferentes entre ellas y con respecto al NR 51. El presente estudio confirma que el empleo de ISSR permite identificar variabilidad genética en plantas regeneradas in vitro de buffelgrass. Fil: Carloni, Edgardo. Instituto Nacional de Tecnología Agropecuaria (Argentina) Fil: López Colomba, Eliana. Instituto Nacional de Tecnología Agropecuaria (Argentina) Fil: Ribotta, Andrea. Instituto Nacional de Tecnología Agropecuaria (Argentina) Fil: Quiroga, Mariana. Instituto Nacional de Tecnología Agropecuaria (Argentina) Fil: Tommasino, Exequiel. Instituto Nacional de Tecnología Agropecuaria (Argentina) Fil: Griffa, Sabrina. Instituto Nacional de Tecnología Agropecuaria (Argentina) Fil: Grunberg, Karina. Buenos Aires (Argentina). Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias 78 Revista de la Facultad de Ciencias Agrarias Vol. 50, no. 2 Revista de la Facultad de Ciencias Agrarias, Vol. 50, no. 2 2018-12-18 eng Inglés http://bdigital.uncu.edu.ar/11479 info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar/ article info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias http://bdigital.uncu.edu.ar/11586 |
| spellingShingle | Variación genética Genética vegetal Regeneración in vitro Cenchrus ciliaris Marcadores genéticos Cultivo in vitro Apomixis Embriogénesis somática Variación somaclonal Cultivo de anteras Diversidad genética como recurso Apomixis Somatic embryogenesis Anther culture ISSR Genetic diversity Análisis de variabilidad Buffelgrass Carloni, Edgardo López Colomba, Eliana Ribotta, Andrea Quiroga, Mariana Tommasino, Exequiel Griffa, Sabrina Grunberg, Karina Analysis of genetic variability in vitro regenerated buffelgrass plants through ISSR molecular markers |
| title | Analysis of genetic variability in vitro regenerated buffelgrass plants through ISSR molecular markers
|
| title_full | Analysis of genetic variability in vitro regenerated buffelgrass plants through ISSR molecular markers
|
| title_fullStr | Analysis of genetic variability in vitro regenerated buffelgrass plants through ISSR molecular markers
|
| title_full_unstemmed | Analysis of genetic variability in vitro regenerated buffelgrass plants through ISSR molecular markers
|
| title_short | Analysis of genetic variability in vitro regenerated buffelgrass plants through ISSR molecular markers
|
| title_sort | analysis of genetic variability in vitro regenerated buffelgrass plants through issr molecular markers |
| topic | Variación genética Genética vegetal Regeneración in vitro Cenchrus ciliaris Marcadores genéticos Cultivo in vitro Apomixis Embriogénesis somática Variación somaclonal Cultivo de anteras Diversidad genética como recurso Apomixis Somatic embryogenesis Anther culture ISSR Genetic diversity Análisis de variabilidad Buffelgrass |
| url | http://bdigital.uncu.edu.ar/11586 |