| Sumario: | Trichloris crinita is among the most important native forage grasses in arid regions
of America. Despite its importance, molecular resources and sequence data are
extremely scarce in this species. In the present study, SSR markers were developed
using available DNA sequences from grass taxa phylogenetically-related to Trichloris
(Eleusine coracana, Cynodon dactylon and ‘Cynodon dactylon x Cynodon transvaalensis’).
Marker transferability was evaluated in a panel of eight T. crinita accessions and five
closely-related species. Of the 105 SSR primer pairs evaluated, 16 amplified products
of expected size in T. crinita, whereas transferability to other grass species ranged from
12 (in Chloris castilloniana) to 28 SSRs (in Eleusine coracana). Six of the 16 SSR markers
successfully transferred to T. crinita (37.5%) were polymorphic, and were further used
to assess genetic diversity in eight T. crinita accessions. The analysis revealed a total of
23 SSR alleles (3.83 alleles/locus), allowing the discrimination of all T. crinita accessions,
with pair-wise genetic similarities ranging from 0.35 to 0.81 (Jaccard coefficient). Mean
(and range) values for observed (Ho) and expected heterozygosity (He) were 0.53
(0.0-1.0) and 0.63 (0.48-0.79), respectively.
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