Biblioteca Digital de VanguardiaInduction of callus and adventitious shoots on epicotyl and hypocotyl segments of cumaru (Dipteryx odorata)
Somatic embryogenesis from callus induced in epicotyl and hypocotyl segments can be viable native species in order to better -benefit ratio costs, and rates of clonal multiplication. In this sense, two trials were established to induce callus and adventitious buds on hypocotyl and epicotyl segments...
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| Formato: | info:eu-repo/semantics/article |
| Lenguaje: | Inglés |
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Federal University of Piauí
2018
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| Acceso en línea: | https://comunicatascientiae.com.br/comunicata/article/view/1618 http://biblioteca-repositorio.clacso.edu.ar/handle/CLACSO/77834 |
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| author | Sampaio, Paulo Tarso Barbosa Jardim, Lyana Silva Blind, Ariel Dotto Bruno, Flavio Mauro Souza |
| author_facet | Sampaio, Paulo Tarso Barbosa Jardim, Lyana Silva Blind, Ariel Dotto Bruno, Flavio Mauro Souza |
| author_sort | Sampaio, Paulo Tarso Barbosa |
| collection | Repositorio |
| description | Somatic embryogenesis from callus induced in epicotyl and hypocotyl segments can be viable native species in order to better -benefit ratio costs, and rates of clonal multiplication. In this sense, two trials were established to induce callus and adventitious buds on hypocotyl and epicotyl segments of cumaru bean seedlings germinated in vitro in different concentrations and combinations of growth regulators. At first, we used the MS medium supplementwith ANA (0.0, 1.5 mg.L-1) and TDZ (0.0, 4.0 and 8.0 mg.L-1) distributed in factorial 2 x 3 x 2 (x auxin cytokinin x explant) with eight replications. In the second, it was used the WPM medium supplemented with BAP (2.0 mg L-1) and plus 2,4-D (2.0 and 4.0 mg L-1) in a factorial 2 x 2 (auxin x explant) with 15 repetitions each. They were evaluating callus formation and the average number of adventitious shoots during the period of 90 days. The results indicated that the highest average for callus formation was observed when the explants were subjected to concentrations of 8.0 mg L-1 TDZ combined with 1.5 mg L-1 ANA in MS medium. For the formation of buds, the WPM medium plus 2.0 mg L-1 2,4-D in the second experiment, induced higher number of shoots, being significant the use of auxin, and its interaction with the type of explant. |
| format | info:eu-repo/semantics/article |
| id | clacso-CLACSO77834 |
| institution | CLACSO, Repositorio Digital |
| language | Inglés |
| publishDate | 2018 |
| publisher | Federal University of Piauí |
| record_format | greenstone |
| spelling | clacso-CLACSO778342022-03-21T20:07:53Z Induction of callus and adventitious shoots on epicotyl and hypocotyl segments of cumaru (Dipteryx odorata) Induction of callus and adventitious shoots on epicotyl and hypocotyl segments of cumaru (Dipteryx odorata) Sampaio, Paulo Tarso Barbosa Jardim, Lyana Silva Blind, Ariel Dotto Bruno, Flavio Mauro Souza calogênese Cumaru Dipteryx odorata (Aubl.) Willd Somatic embryogenesis from callus induced in epicotyl and hypocotyl segments can be viable native species in order to better -benefit ratio costs, and rates of clonal multiplication. In this sense, two trials were established to induce callus and adventitious buds on hypocotyl and epicotyl segments of cumaru bean seedlings germinated in vitro in different concentrations and combinations of growth regulators. At first, we used the MS medium supplementwith ANA (0.0, 1.5 mg.L-1) and TDZ (0.0, 4.0 and 8.0 mg.L-1) distributed in factorial 2 x 3 x 2 (x auxin cytokinin x explant) with eight replications. In the second, it was used the WPM medium supplemented with BAP (2.0 mg L-1) and plus 2,4-D (2.0 and 4.0 mg L-1) in a factorial 2 x 2 (auxin x explant) with 15 repetitions each. They were evaluating callus formation and the average number of adventitious shoots during the period of 90 days. The results indicated that the highest average for callus formation was observed when the explants were subjected to concentrations of 8.0 mg L-1 TDZ combined with 1.5 mg L-1 ANA in MS medium. For the formation of buds, the WPM medium plus 2.0 mg L-1 2,4-D in the second experiment, induced higher number of shoots, being significant the use of auxin, and its interaction with the type of explant. Somatic embryogenesis from callus induced in epicotyl and hypocotyl segments can be viable native species in order to better -benefit ratio costs, and rates of clonal multiplication. In this sense, two trials were established to induce callus and adventitious buds on hypocotyl and epicotyl segments of cumaru bean seedlings germinated in vitro in different concentrations and combinations of growth regulators. At first, we used the MS medium supplementwith ANA (0.0, 1.5 mg.L-1) and TDZ (0.0, 4.0 and 8.0 mg.L-1) distributed in factorial 2 x 3 x 2 (x auxin cytokinin x explant) with eight replications. In the second, it was used the WPM medium supplemented with BAP (2.0 mg L-1) and plus 2,4-D (2.0 and 4.0 mg L-1) in a factorial 2 x 2 (auxin x explant) with 15 repetitions each. They were evaluating callus formation and the average number of adventitious shoots during the period of 90 days. The results indicated that the highest average for callus formation was observed when the explants were subjected to concentrations of 8.0 mg L-1 TDZ combined with 1.5 mg L-1 ANA in MS medium. For the formation of buds, the WPM medium plus 2.0 mg L-1 2,4-D in the second experiment, induced higher number of shoots, being significant the use of auxin, and its interaction with the type of explant. 2018-11-04 2022-03-21T20:07:53Z 2022-03-21T20:07:53Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artigo Cientifico https://comunicatascientiae.com.br/comunicata/article/view/1618 10.14295/cs.v9i3.1618 http://biblioteca-repositorio.clacso.edu.ar/handle/CLACSO/77834 eng https://comunicatascientiae.com.br/comunicata/article/view/1618/580 Copyright (c) 2018 Paulo Tarso Barbosa Sampaio, Lyana Silva Jardim, Ariel Dotto Blind, Flavio Mauro Souza Bruno application/pdf Federal University of Piauí Comunicata Scientiae; Vol. 9 No. 3 (2018); 475-480 Comunicata Scientiae; v. 9 n. 3 (2018); 475-480 2177-5133 2176-9079 |
| spellingShingle | calogênese Cumaru Dipteryx odorata (Aubl.) Willd Sampaio, Paulo Tarso Barbosa Jardim, Lyana Silva Blind, Ariel Dotto Bruno, Flavio Mauro Souza Induction of callus and adventitious shoots on epicotyl and hypocotyl segments of cumaru (Dipteryx odorata) |
| title | Induction of callus and adventitious shoots on epicotyl and hypocotyl segments of cumaru (Dipteryx odorata) |
| title_full | Induction of callus and adventitious shoots on epicotyl and hypocotyl segments of cumaru (Dipteryx odorata) |
| title_fullStr | Induction of callus and adventitious shoots on epicotyl and hypocotyl segments of cumaru (Dipteryx odorata) |
| title_full_unstemmed | Induction of callus and adventitious shoots on epicotyl and hypocotyl segments of cumaru (Dipteryx odorata) |
| title_short | Induction of callus and adventitious shoots on epicotyl and hypocotyl segments of cumaru (Dipteryx odorata) |
| title_sort | induction of callus and adventitious shoots on epicotyl and hypocotyl segments of cumaru (dipteryx odorata) |
| topic | calogênese Cumaru Dipteryx odorata (Aubl.) Willd |
| url | https://comunicatascientiae.com.br/comunicata/article/view/1618 http://biblioteca-repositorio.clacso.edu.ar/handle/CLACSO/77834 |