Relationship of Insecticidal Activity and Resistance to Proteolysis of Two Glucose/ Mannose Plant Lectins

Purified seed lectins from the legumes Canavalia brasiliensis (ConBr) and Cratylia floribunda (CFL)were tested for insecticidal activity against Callosobruchus maculatus (Coleoptera: Bruchidae)and Dysdercus peruvianus (Hemiptera: Pyrrhocoridae). ConBr and CFL caused negative effects onlarvae of C. m...

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Detalles Bibliográficos
Autores principales: Freitas, Cleverson Diniz, Ramos, Marcio Viana, Souza, Diego Pereira, Marinho-Filho, Jose Delano Barreto, Teixeira, Fabiano Moura, Oliveira, Jefferson Soares
Formato: info:eu-repo/semantics/article
Lenguaje:Portugués
Publicado: Federal University of Piauí 2011
Materias:
Acceso en línea:https://comunicatascientiae.com.br/comunicata/article/view/15
http://biblioteca-repositorio.clacso.edu.ar/handle/CLACSO/77477
Descripción
Sumario:Purified seed lectins from the legumes Canavalia brasiliensis (ConBr) and Cratylia floribunda (CFL)were tested for insecticidal activity against Callosobruchus maculatus (Coleoptera: Bruchidae)and Dysdercus peruvianus (Hemiptera: Pyrrhocoridae). ConBr and CFL caused negative effects onlarvae of C. maculatus at levels of 1% and 2% (w/w). Time of development of C. maculatus insects,grown in artificial seeds containing both lectins, was not significantly altered, but the emergencewas slightly diminished. Both lectins were resisted to proteolysis by gut proteases of C. maculatuslarvae. Artificial diets containing 1% and 2% of ConBr or CFL caused no effect on weight and survivalof D. peruvianus nymphs. Gut proteases of D. peruvianus were able to breakdown ConBr and CFLafter 2 hours of incubation. The ability of insects to digest the proteins was correlated directly withthe deleterious effect observed.